Product Information

Property	Description
Product Name	Anti-mouse CAR Recombinant Monoclonal Antibody
Antibody ID	MM0006
Target Protein	Constitutive androstane receptor (CAR)
UniProt Accession	O35627-1 / O35627-2
Molecular Weight	Isoform 1: 40.9 kDa; Isoform 2: 32.5 kDa
Antibody Type	Recombinant monoclonal antibody
Host Species	Mouse
Recombinant Format	Full-length mouse IgG1
Isotype	Mouse IgG1
Species Reactivity	Mouse validated; human CAR cross-reactive
Tested Applications	WB, IF, ELISA
Recommended Dilution for WB	1:500–1:5000
Antigen	Recombinant epitope-Fc fusion protein
Epitope Sequence	MTAMLTLETMASEEEYGP
Expression System	HEK293 cells
Purification	Protein G affinity chromatography
Conjugate	Unconjugated
Concentration	0.1 mg/mL
Storage Buffer	0.1 M Tris, 0.05 M Glycine, 0.07 M NaCl, 2 g/L BSA, 50% glycerol, pH 7
Storage Condition	Store at -20°C
Research Use	For research use only. Not for diagnostic or therapeutic applications.

Product Background

Constitutive androstane receptor (CAR) is a ligand-activated nuclear receptor involved in xenobiotic metabolism, drug detoxification, lipid metabolism, and hepatic gene regulation. CAR plays an important role in regulation of cytochrome P450 enzymes, liver metabolism, inflammation, and toxicological responses. CAR is widely studied in pharmacology, metabolism, toxicology, and liver biology research.

Antibody Development

MM0006 was generated by phage-display selection using a mouse-derived antibody library. Recombinant epitope-Fc fusion protein containing the target epitope sequence was used as the selection antigen. Following identification of the initial antibody candidate, structure-guided affinity optimization was performed to improve binding affinity and specificity.

The optimized antibody was reconstructed as a full-length mouse IgG1 recombinant antibody and expressed in HEK293 cells.

Binding and specificity were evaluated by:

• ELISA using recombinant epitope-Fc fusion antigen
• Western blot detection of endogenous mouse CAR protein in mammalian tissue and cell lysates
• Immunofluorescence staining of endogenous CAR in mouse brain tissue
• Cross-reactivity analysis demonstrating recognition of human CAR protein

Validation Data

ELISA Validation

ELISA demonstrated specific binding to recombinant epitope-Fc fusion antigen, while minimal binding was observed against Fc control protein.

Validation conditions:

• Well 1: Fc control protein
• Well 2: epitope-Fc fusion protein
• Detection substrate: TMB

Note: ELISA validation confirms binding to the epitope-Fc fusion antigen. Full-length CAR protein binding in solution has not been tested by ELISA.

Western Blot Validation – Endogenous Protein

Western blot analysis detected endogenous CAR protein in mammalian tissue and cell lysates under reducing conditions.

Validated samples:

• HEK293T cells
• Lung tissue
• Brain tissue

Detection condition:

• Standard reducing Western blot
• HRP-conjugated anti-mouse secondary antibody

MM0006 also demonstrated cross-reactivity with human CAR protein.

Immunofluorescence Validation

Immunofluorescence staining of mouse brain tissue showed endogenous CAR-associated signal using MM0006. CAR signal is shown in green and nuclei are counterstained with DAPI (blue).