Anti-mouse GPR20 (Extracellular) Recombinant Monoclonal Antibody (MM0060)
Price range: $230.00 through $720.00
Anti-mouse GPR20 Recombinant Monoclonal Antibody (MM0060) is a high-affinity recombinant monoclonal antibody generated by phage-display selection from a mouse-derived antibody library, followed by structure-guided affinity optimization. The antibody recognizes an epitope (AA 1-21) located within the N-terminal extracellular flexible region of G protein-coupled receptor 20 (GPR20). It has been validated by ELISA using a recombinant epitope-Fc fusion antigen, by Western blot against a recombinant MBP-epitope fusion protein, by Western blot detection of endogenous GPR20 protein in mouse tissue lysates, and by immunofluorescence staining of mouse liver tissue.
Also known as: mouse GPR20, G-protein coupled receptor 20, G protein-coupled receptor 20.
For research use only. Not for diagnostic or therapeutic applications.
Product Information
| Property | Description |
|---|---|
| Product Name | Anti-mouse GPR20 Recombinant Monoclonal Antibody |
| Antibody ID | MM0060 |
| Target Protein | G-protein coupled receptor 20 |
| Target Protein Short Name | mGPR20 |
| UniProt Accession | Q8BYC4 |
| Molecular Weight | Mouse: 39.4 kDa |
| Antibody Type | Recombinant monoclonal antibody |
| Host Species | Mouse |
| Recombinant Format | Full-length mouse IgG1 |
| Isotype | Mouse IgG1 |
| Species Reactivity | Mouse |
| Tested Applications | WB, IF |
| Recommended Dilution for WB | 1:500–1:5000 |
| Antigen | Recombinant epitope-Fc fusion protein |
| Epitope Sequence | MPSALSMRPWDAALPNTTAAA |
| Expression System | HEK293 cells |
| Purification | Protein G affinity chromatography |
| Conjugate | Unconjugated |
| Concentration | 0.1 mg/mL |
| Storage Buffer | 0.1 M Tris, 0.05 M Glycine, 0.07 M NaCl, 2 g/L BSA, 50% glycerol, pH 7 |
| Storage Condition | Store at −20°C |
| Research Use | For research use only. Not for diagnostic or therapeutic applications. |
Product Background
G-protein coupled receptor 20 (GPR20) is a member of the Class A rhodopsin-like G protein-coupled receptor (GPCR) family. As an orphan GPCR, its endogenous ligand and physiological signaling mechanisms remain incompletely understood. GPR20 is expressed in multiple mouse tissues, including the liver, intestine, and brain, and is believed to participate in receptor-mediated signal transduction, cellular communication, and tissue-specific physiological regulation. Ongoing studies continue to investigate its potential roles in GPCR biology, metabolism, and organ homeostasis.
GPR20 is a useful target for researchers studying GPCR biology, membrane receptor expression, signal transduction, tissue distribution, receptor pharmacology, metabolism, and cell signaling. Reliable antibodies capable of detecting endogenous GPR20 protein are valuable tools for investigating its biological function and expression in normal physiology and disease models.
Antibody Development
MM0060 was generated by phage-display selection using a mouse-derived antibody library. A recombinant epitope-Fc fusion protein containing the GPR20 epitope sequence was used as the selection antigen. After identification of the initial antibody candidate, structure-guided affinity optimization was performed to improve binding affinity and specificity.
The optimized antibody was reconstructed as a full-length mouse IgG1 recombinant antibody and expressed in HEK293 cells.
Binding and specificity were evaluated by:
- ELISA using recombinant epitope-Fc fusion antigen
- Western blot using recombinant MBP-epitope fusion protein
- Western blot detection of endogenous mouse GPR20 protein
- Immunofluorescence staining of mouse liver tissue
Validation Data
ELISA Validation
ELISA demonstrated specific binding to recombinant epitope-Fc fusion antigen, while minimal binding was observed against Fc-only control protein.
Validation conditions:
- Fc control protein
- Epitope-Fc fusion protein
- Detection substrate: TMB
Note: ELISA validation confirms binding to the recombinant epitope-Fc fusion antigen. Full-length GPR20 protein binding in solution has not been evaluated by ELISA.
Western Blot Validation – Recombinant Fusion Protein
Western blot analysis confirmed specific recognition of recombinant MBP-epitope fusion protein, with minimal signal against MBP control protein.
Validation conditions:
- MBP control protein
- MBP-epitope fusion protein
- Standard reducing Western blot
Western Blot Validation – Endogenous Full-Length Protein
Western blot analysis detected endogenous mouse GPR20 protein in mouse tissue lysates under reducing conditions.
Validated samples:
- Mouse liver
- Mouse brain
- Mouse intestine
Observed bands:
- Major immunoreactive bands were detected around 52 kDa.
- Additional bands may represent receptor processing intermediates, post-translationally modified forms, degradation products, or receptor-associated protein complexes.
Immunofluorescence Validation
Immunofluorescence staining demonstrated endogenous GPR20 expression in mouse liver tissue.
Validation conditions:
- Mouse liver tissue
- GPR20 (green)
- DAPI nuclear counterstain (blue)
Representative staining showed specific GPR20 immunoreactivity within liver tissue architecture, supporting the suitability of MM0060 for immunofluorescence applications.
| Size | 20 ul, 100ul |
|---|
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