Product Information

Property	Description
Product Name	Anti-human LXRα Recombinant Monoclonal Antibody
Antibody ID	HM0023
Target Protein	Oxysterols receptor LXR-alpha (LXRα)
UniProt Accession	Q13133-1 / Q13133-2
Molecular Weight	Isoform 1: 50.4 kDa; Isoform 2: 43.6 kDa
Antibody Type	Recombinant monoclonal antibody
Host Species	Mouse
Recombinant Format	Full-length mouse IgG1
Isotype	Mouse IgG1
Species Reactivity	Human validated only
Tested Applications	WB, ELISA
Recommended Dilution for WB	1:500–1:5000
Antigen	Human-derived recombinant epitope-Fc fusion protein
Epitope Sequence	DIPPDSAVELWKPGAQDASSQA
Expression System	HEK293 cells
Purification	Protein G affinity chromatography
Conjugate	Unconjugated
Concentration	0.1 mg/mL
Storage Buffer	0.1 M Tris, 0.05 M Glycine, 0.07 M NaCl, 2 g/L BSA, 50% glycerol, pH 7
Storage Condition	Store at -20°C
Research Use	For research use only. Not for diagnostic or therapeutic applications.

Product Background

Liver X receptor alpha (LXRα, NR1H3) is a ligand-activated nuclear receptor involved in cholesterol metabolism, lipid homeostasis, inflammatory signaling, and metabolic regulation. Human LXRα is widely studied in liver biology, cardiovascular disease, lipid metabolism, and inflammatory signaling pathways. Due to its important role in cholesterol transport and metabolic regulation, LXRα is an important target in metabolism and translational research.

Also known as: hLXRa, hLXRα, Human LXRa, Human LXRα, Human LXR alpha, LXRa, LXRα, LXR alpha, Liver X receptor alpha, Liver X receptor-alpha, Oxysterols receptor LXR-alpha, Oxysterols receptor alpha, and NR1H3.

Antibody Development

HM0023 was generated by phage-display selection using a mouse-derived antibody library. A human-derived recombinant epitope-Fc fusion protein containing the LXRα epitope sequence was used as the selection antigen. Following identification of the initial antibody candidate, structure-guided affinity optimization was performed to improve binding affinity and specificity.

The optimized antibody was reconstructed as a full-length mouse IgG1 recombinant antibody and expressed in HEK293 cells.

Binding and specificity were evaluated by:

• ELISA using human-derived recombinant epitope-Fc fusion antigen
• Western blot using recombinant MBP-epitope fusion protein
• Western blot detection of endogenous human LXRα protein in mammalian cell lysates

Validation Data

ELISA Validation

ELISA demonstrated specific binding to the human-derived recombinant epitope-Fc fusion antigen, while minimal binding was observed against Fc control protein.

Validation conditions:

• Well 1: Fc control protein
• Well 2: Epitope-Fc fusion protein
• Detection substrate: TMB

Note: ELISA validation confirms binding to the epitope-Fc fusion antigen. Full-length LXRα protein binding in solution has not been tested by ELISA.

Western Blot Validation – Recombinant Fusion Protein

Western blot analysis demonstrated specific recognition of recombinant MBP-epitope fusion protein expressed in BL21(DE3) cells, while no significant binding was observed against MBP control protein.

Validation conditions:

• Lane 1: MBP control protein
• Lane 2: MBP-epitope fusion protein
• Standard reducing Western blot

Western Blot Validation – Endogenous Full-Length Protein

Western blot analysis detected endogenous human LXRα protein in mammalian tissue and cell lysates under reducing conditions.

Validated samples:

• HEK293T cells
• HeLa cells
• HepG2 cells

Detection condition:

• Standard reducing Western blot
• HRP-conjugated anti-mouse secondary antibody