Anti-mouse/human PDGFRA / CD140a Recombinant Monoclonal Antibody (XM0046)
Price range: $180.00 through $480.00
Anti-mouse/human PDGFRA / CD140a Recombinant Monoclonal Antibody (XM0046) is a high-affinity recombinant monoclonal antibody generated by phage-display selection from a mouse-derived antibody library, followed by structure-guided affinity optimization. The antibody recognizes both mouse and human PDGFRα (CD140a) through a conserved epitope (AA 747–767) located within an intracellular flexible region of platelet-derived growth factor receptor alpha (PDGFRα, also known as CD140a). It has been validated by ELISA using a recombinant epitope-Fc fusion antigen, by Western blot against a recombinant MBP-epitope fusion protein, by Western blot detection of endogenous PDGFRα protein in HEK293T cell lysates and mouse tissue lysates, and by immunofluorescence staining of mouse skin tissue.
Also known as: PDGFRA, CD140a, PDGFR alpha, PDGFRα, and Platelet-derived growth factor receptor alpha.
For research use only. Not for diagnostic or therapeutic applications.
Product Information
| Property | Description |
|---|---|
| Product Name | Anti-mouse/human PDGFRA / CD140a Recombinant Monoclonal Antibody |
| Antibody ID | XM0046 |
| Target Protein | Platelet-derived growth factor receptor alpha (CD140a / PDGFRα) |
| UniProt Accession | Human: P16234-1; Mouse: P26618-1, P26618-2 |
| Molecular Weight | Human: 122.7 kDa; Mouse: 88.6–122.7 kDa |
| Antibody Type | Recombinant monoclonal antibody |
| Host Species | Mouse |
| Recombinant Format | Full-length mouse IgG1 |
| Isotype | Mouse IgG1 |
| Species Reactivity | Mouse and human |
| Tested Applications | WB, IF, ELISA |
| Recommended Dilution for WB | 1:500–1:5000 |
| Antigen | Recombinant epitope-Fc fusion protein |
| Epitope Sequence | ERKEVSKYSDIQRSLYDRPAS |
| Expression System | HEK293 cells |
| Purification | Protein G affinity chromatography |
| Conjugate | Unconjugated |
| Concentration | 0.1 mg/mL |
| Storage Buffer | 0.1 M Tris, 0.05 M Glycine, 0.07 M NaCl, 2 g/L BSA, 50% glycerol, pH 7 |
| Storage Condition | Store at -20°C. |
| Research Use | For research use only. Not for diagnostic or therapeutic applications. |
Product Background
Platelet-derived growth factor receptor alpha (PDGFRα / CD140a) is a receptor tyrosine kinase involved in cell proliferation, differentiation, migration, tissue development, fibrosis, and tumorigenesis. PDGFRα signaling regulates multiple biological processes including mesenchymal cell development, stem cell biology, wound healing, and oncogenic signaling pathways.
CD140a is widely studied in cancer biology, fibrosis research, stem cell biology, developmental biology, and receptor tyrosine kinase signaling.
Antibody Development
XM0046 was generated by phage-display selection using a mouse-derived antibody library. Recombinant epitope-Fc fusion protein containing the CD140a epitope sequence was used as the selection antigen. Following identification of the initial antibody candidate, structure-guided affinity optimization was performed to improve binding affinity and specificity.
The optimized antibody was reconstructed as a full-length mouse IgG1 recombinant antibody and expressed in HEK293 cells.
Binding and specificity were evaluated by:
- ELISA using recombinant epitope-Fc fusion antigen
- Western blot using recombinant MBP-epitope fusion protein
- Western blot detection of endogenous CD140a / PDGFRα protein in mouse tissues
- Immunofluorescence staining of endogenous mouse CD140a (PDGFRα) in skin tissue
Validation Data
ELISA Validation
ELISA demonstrated specific binding to recombinant epitope-Fc fusion antigen, while minimal binding was observed against Fc control protein.
Validation conditions:
- Well 1: Fc control protein
- Well 2: Epitope-Fc fusion protein
- Detection substrate: TMB
Note:
ELISA validation confirms binding to the epitope-Fc fusion antigen. Full-length CD140a protein binding in solution has not been tested by ELISA.
Western Blot Validation – Recombinant Fusion Protein
Western blot analysis demonstrated specific recognition of recombinant MBP-epitope fusion protein expressed in BL21(DE3) cells, while no significant binding was observed against MBP control protein.
Validation conditions:
- Lane 1: MBP control protein
- Lane 2: MBP-epitope fusion protein
- Standard reducing Western blot
Western Blot Validation – Endogenous Full-Length Protein
Western blot analysis detected endogenous CD140a / PDGFRα protein in mouse heart and kidney tissues under reducing conditions.
Validated samples:
- Mouse heart tissue
- Mouse kidney tissue
Detection condition:
- Standard reducing Western blot
- HRP-conjugated anti-mouse secondary antibody
Observed bands:
- Major bands observed near expected PDGFRα molecular weights
- Additional lower molecular weight bands may represent processed protein species or isoforms
Immunofluorescence Validation
Immunofluorescence staining of mouse skin tissue showed endogenous CD140a-associated signal using XM0046. CD140a signal is shown in green and nuclei are counterstained with DAPI (blue)
| Size | 20 ul, 100 ul |
|---|
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