Anti-mouse SAV1 Recombinant Monoclonal Antibody (MM0034)
Price range: $180.00 through $480.00
Anti-mouse SAV1 Recombinant Monoclonal Antibody (MM0034) is a high-affinity recombinant monoclonal antibody generated by phage-display selection from a mouse-derived antibody library, followed by structure-guided affinity optimization. The antibody recognizes an epitope (AA 124–145) located within an intrinsically disordered region of protein salvador homolog 1 (SAV1). It has been validated by ELISA using a recombinant epitope-Fc fusion antigen, by Western blot against a recombinant MBP-epitope fusion protein, by Western blot detection of endogenous SAV1 protein in HEK293T cell lysates and mouse tissue lysates, and by immunofluorescence staining of mouse liver tissue. The antibody also cross-reacts with human SAV1.
Also known as: mSAV1, SAV1, WW45, and Protein salvador homolog 1.
For research use only. Not for diagnostic or therapeutic applications.
Product Information
| Property | Description |
|---|---|
| Product Name | Anti-mouse SAV1 Recombinant Monoclonal Antibody |
| Antibody ID | MM0034 |
| Target Protein | Protein salvador homolog 1 |
| Target Protein Short Name | SAV1 |
| UniProt Accession | Q8VEB2 |
| Molecular Weight | 44.8 kDa |
| Antibody Type | Recombinant monoclonal antibody |
| Host Species | Mouse |
| Recombinant Format | Full-length mouse IgG1 |
| Isotype | Mouse IgG1 |
| Species Reactivity | Mouse, human |
| Tested Applications | WB, IF, ELISA |
| Recommended Dilution for WB | 1:500–1:5000 |
| Antigen | Recombinant epitope-Fc fusion protein |
| Epitope Sequence | AVENGDSGSRYFFSDNFFDGQR |
| Expression System | HEK293 cells |
| Purification | Protein G affinity chromatography |
| Conjugate | Unconjugated |
| Concentration | 0.1 mg/mL |
| Storage Buffer | 0.1 M Tris, 0.05 M Glycine, 0.07 M NaCl, 2 g/L BSA, 50% glycerol, pH 7 |
| Storage Condition | Store at -20°C |
| Research Use | For research use only. Not for diagnostic or therapeutic applications |
Product Background
Protein salvador homolog 1 (SAV1, Salvador family WW domain-containing protein 1) is a core scaffold protein within the Hippo signaling pathway. SAV1 interacts with MST kinases and contributes to regulation of cell proliferation, apoptosis, tissue homeostasis, organ size control, and tumor suppression.
SAV1 is broadly studied in cancer biology, Hippo pathway signaling, stem cell biology, tissue regeneration, developmental biology, and signal transduction research.
Antibody Development
MM0034 was generated by phage-display selection using a mouse-derived antibody library. Recombinant epitope-Fc fusion protein containing the SAV1 epitope sequence was used as the selection antigen. Following identification of the initial antibody candidate, structure-guided affinity optimization was performed to improve binding affinity and specificity.
The optimized antibody was reconstructed as a full-length mouse IgG1 recombinant antibody and expressed in HEK293 cells.
Binding and specificity were evaluated by:
- ELISA using recombinant epitope-Fc fusion antigen
- Western blot using recombinant MBP-epitope fusion protein
- Western blot detection of endogenous full-length SAV1 protein
- Immunofluorescence staining of endogenous mouse SAV1 in brain tissue
Validation Data
ELISA Validation
ELISA demonstrated specific binding to recombinant epitope-Fc fusion antigen, while minimal binding was observed against Fc control protein.
Validation conditions:
- Well 1: Fc control protein
- Well 2: Epitope-Fc fusion protein
- Detection substrate: TMB
Note:
ELISA validation confirms binding to the epitope-Fc fusion antigen. Full-length SAV1 protein binding in solution has not been tested by ELISA.
Western Blot Validation – Recombinant Fusion Protein
Western blot analysis demonstrated specific recognition of recombinant MBP-epitope fusion protein expressed in BL21(DE3) cells, while minimal binding was observed against MBP control protein.
Validation conditions:
- Lane 1: MBP control protein
- Lane 2: MBP-epitope fusion protein
- Standard reducing Western blot
Western Blot Validation – Endogenous Full-Length Protein
Western blot analysis detected endogenous full-length SAV1 protein under reducing conditions.
Validated samples:
- Mouse brain tissue lysate
- Mouse liver tissue lysate
- Human HEK293T (293T) cells
Observed bands:
- Major immunoreactive bands were detected near the expected molecular weight range for endogenous SAV1 protein.
- Additional bands may represent SAV1-associated complexes, post-translationally modified forms, proteolytic fragments, or tissue-specific processing products.
The antibody also recognizes human SAV1, likely due to high conservation of the epitope sequence between mouse and human proteins (AVENGDSGSRYYYSDNFFDGQR).
Detection condition:
- Standard reducing Western blot
- HRP-conjugated anti-mouse secondary antibody
Immunofluorescence Validation
Immunofluorescence staining of mouse brain tissue showed endogenous SAV1-associated signal using MM0034. SAV1 signal is shown in green and nuclei are counterstained with DAPI (blue).
| Size | 20 ul, 100 ul |
|---|
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