Product Information

Property	Description
Product Name	Anti-mouse/human ERRα Recombinant Monoclonal Antibody
Antibody ID	XM0022
Target Protein	Estrogen-related receptor alpha (ERRα)
UniProt Accession	Human: P11474-1 / P11474-2; Mouse: O08580
Molecular Weight	Human isoforms: 45.4–45.5 kDa; Mouse: 45.5 kDa
Antibody Type	Recombinant monoclonal antibody
Host Species	Mouse
Recombinant Format	Full-length mouse IgG1
Isotype	Mouse IgG1
Species Reactivity	Mouse and human validated
Tested Applications	WB, IF, ELISA
Recommended Dilution for WB	1:500–1:5000
Antigen	Recombinant epitope-Fc fusion protein
Epitope Sequence	GIEPLYIKAEPASPDSPKG
Expression System	HEK293 cells
Purification	Protein G affinity chromatography
Conjugate	Unconjugated
Concentration	0.1 mg/mL
Storage Buffer	0.1 M Tris, 0.05 M Glycine, 0.07 M NaCl, 2 g/L BSA, 50% glycerol, pH 7
Storage Condition	Store at -20°C.
Research Use	For research use only. Not for diagnostic or therapeutic applications.

Product Background

Estrogen-related receptor alpha (ERR1 / ESRRa) is an orphan nuclear receptor involved in mitochondrial metabolism, oxidative phosphorylation, lipid metabolism, energy homeostasis, and transcriptional regulation. ERR1 is widely studied in metabolism, endocrinology, cancer biology, mitochondrial biology, and skeletal muscle physiology. Because ERR1 plays a central role in cellular energy regulation, it is an important target in metabolic and translational research.

Antibody Development

XM0022 was generated by phage-display selection using a mouse-derived antibody library. Recombinant epitope-Fc fusion protein containing the ERR1 epitope sequence was used as the selection antigen. Following identification of the initial antibody candidate, structure-guided affinity optimization was performed to improve binding affinity and specificity.

The optimized antibody was reconstructed as a full-length mouse IgG1 recombinant antibody and expressed in HEK293 cells.

Binding and specificity were evaluated by:

• ELISA using recombinant epitope-Fc fusion antigen
• Western blot using recombinant MBP-epitope fusion protein
• Western blot detection of endogenous ERR1 protein in mouse tissues and human cells
• Immunofluorescence staining of endogenous mouse ERR1 in kidney tissue

Validation Data

ELISA Validation

ELISA demonstrated specific binding to recombinant epitope-Fc fusion antigen, while minimal binding was observed against Fc control protein.

Validation conditions:

• Well 1: Fc control protein
• Well 2: epitope-Fc fusion protein
• Detection substrate: TMB

Note: ELISA validation confirms binding to the epitope-Fc fusion antigen. Full-length ERR1 protein binding in solution has not been tested by ELISA.

Western Blot Validation – Recombinant Fusion Protein

Western blot analysis demonstrated specific recognition of recombinant MBP-epitope fusion protein expressed in BL21(DE3) cells, while no significant binding was observed against MBP control protein.

Validation conditions:

• Lane 1: MBP control protein
• Lane 2: MBP-epitope fusion protein
• Standard reducing Western blot

Western Blot Validation – Endogenous Full-Length Protein

Western blot analysis detected endogenous ERR1 protein in human 293T cells, HepG2 cells, and mouse kidney tissue under reducing conditions.

Validated samples:

• 293T cells
• Mouse kidney tissue
• HepG2 cells

Detection condition:

• Standard reducing Western blot
• HRP-conjugated anti-mouse secondary antibody

Immunofluorescence Validation

Immunofluorescence staining of mouse kidney tissue showed endogenous ERR1-associated signal using XM0022. ERR1 signal is shown in green and nuclei are counterstained with DAPI (blue).