Anti-mouse/human RARγ Recombinant Monoclonal Antibody (XM0044)
Price range: $180.00 through $480.00
Anti-mouse/human RARγ Recombinant Monoclonal Antibody (XM0044) is a high-affinity recombinant monoclonal antibody generated by phage-display selection from a mouse-derived antibody library, followed by structure-guided affinity optimization. The antibody recognizes both mouse and human RARγ through a conserved epitope (AA 24–43) located within the N-terminal flexible region of retinoic acid receptor gamma (RARγ). It has been validated by ELISA using a recombinant epitope-Fc fusion antigen, by Western blot against a recombinant MBP-epitope fusion protein, by Western blot detection of endogenous RARγ protein in HEK293T cell lysates and mouse tissue lysates, and by immunofluorescence staining of mouse skin tissue.
Also known as: RARg, RARγ, RAR gamma, and Retinoic acid receptor gamma.
For research use only. Not for diagnostic or therapeutic applications.
Product Information
| Property | Description |
|---|---|
| Product Name | Anti-mouse/human RARγ Recombinant Monoclonal Antibody |
| Antibody ID | XM0044 |
| Target Protein | Retinoic acid receptor gamma (RARγ / RARG) |
| UniProt Accession | Human: P13631-1; Mouse: P18911-1, P20787-1 |
| Molecular Weight | Human: 50.3 kDa; Mouse: 50.9 kDa |
| Antibody Type | Recombinant monoclonal antibody |
| Host Species | Mouse |
| Recombinant Format | Full-length mouse IgG1 |
| Isotype | Mouse IgG1 |
| Species Reactivity | Mouse and human |
| Tested Applications | WB, IF, ELISA |
| Recommended Dilution for WB | 1:500–1:5000 |
| Antigen | Recombinant epitope-Fc fusion protein |
| Epitope Sequence | GFPFAFPGALRGSPPFEMLS |
| Expression System | HEK293 cells |
| Purification | Protein G affinity chromatography |
| Conjugate | Unconjugated |
| Concentration | 0.1 mg/mL |
| Storage Buffer | 0.1 M Tris, 0.05 M Glycine, 0.07 M NaCl, 2 g/L BSA, 50% glycerol, pH 7 |
| Storage Condition | Store at -20°C. |
| Research Use | For research use only. Not for diagnostic or therapeutic applications. |
Product Background
Retinoic acid receptor gamma (RARγ / RARG) is a ligand-dependent nuclear receptor involved in retinoic acid signaling, transcriptional regulation, cellular differentiation, embryonic development, stem cell biology, and cancer-related signaling pathways. RARγ is widely studied in developmental biology, oncology, stem cell regulation, and nuclear receptor signaling research.
RARγ functions as a transcription factor that regulates retinoid-responsive genes and plays important roles in tissue differentiation and cellular homeostasis.
Antibody Development
XM0044 was generated by phage-display selection using a mouse-derived antibody library. Recombinant epitope-Fc fusion protein containing the RARγ epitope sequence was used as the selection antigen. Following identification of the initial antibody candidate, structure-guided affinity optimization was performed to improve binding affinity and specificity.
The optimized antibody was reconstructed as a full-length mouse IgG1 recombinant antibody and expressed in HEK293 cells.
Binding and specificity were evaluated by:
- ELISA using recombinant epitope-Fc fusion antigen
- Western blot using recombinant MBP-epitope fusion protein
- Western blot detection of endogenous RARγ protein in human cells and mouse tissue
- Immunofluorescence staining of endogenous mouse RARγ in kidney tissue
Validation Data
ELISA Validation
ELISA demonstrated specific binding to recombinant epitope-Fc fusion antigen, while minimal binding was observed against Fc control protein.
Validation conditions:
- Well 1: Fc control protein
- Well 2: Epitope-Fc fusion protein
- Detection substrate: TMB
Note:
ELISA validation confirms binding to the epitope-Fc fusion antigen. Full-length RARγ protein binding in solution has not been tested by ELISA.
Western Blot Validation – Recombinant Fusion Protein
Western blot analysis demonstrated specific recognition of recombinant MBP-epitope fusion protein expressed in BL21(DE3) cells, while no significant binding was observed against MBP control protein.
Validation conditions:
- Lane 1: MBP control protein
- Lane 2: MBP-epitope fusion protein
- Standard reducing Western blot
Western Blot Validation – Endogenous Full-Length Protein
Western blot analysis detected endogenous RARγ protein in HeLa cells and mouse heart tissue under reducing conditions.
Validated samples:
- HeLa cells
- Mouse heart tissue
Detection condition:
- Standard reducing Western blot
- HRP-conjugated anti-mouse secondary antibody
Observed bands:
- Observed bands located at around 40Kd.
Immunofluorescence Validation
Immunofluorescence staining of mouse kidney tissue showed endogenous RARγ-associated signal using XM0044. RARγ signal is shown in green and nuclei are counterstained with DAPI (blue).
| Size | 20 ul, 100 ul |
|---|
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