Anti-human ACKR1 (Extracellular) Recombinant Monoclonal Antibody (HM0062)
Price range: $230.00 through $720.00
Anti-human ACKR1 Recombinant Monoclonal Antibody (HM0062) is a high-affinity recombinant monoclonal antibody generated by phage-display selection from a mouse-derived antibody library, followed by structure-guided affinity optimization. The antibody recognizes an epitope (AA 14–35) located within the N-terminal extracellular flexible region of atypical chemokine receptor 1 (ACKR1). It has been validated by ELISA using a recombinant epitope-Fc fusion antigen, by Western blot against a recombinant MBP-epitope fusion protein, and by Western blot detection of endogenous ACKR1 protein in human cell lysates.
Also known as: hACKR1, ACKR1, DARC, CD234, and Duffy antigen receptor for chemokines.
For research use only. Not for diagnostic or therapeutic applications.
Product Information
| Property | Description |
|---|---|
| Product Name | Anti-human ACKR1 Recombinant Monoclonal Antibody |
| Antibody ID | HM0062 |
| Target Protein | Atypical chemokine receptor 1 (ACKR1 / DARC) |
| UniProt Accession | Human: Q16570-1, Q16570-2 |
| Molecular Weight | Human: 35.6 kDa |
| Antibody Type | Recombinant monoclonal antibody |
| Host Species | Mouse |
| Recombinant Format | Full-length mouse IgG1 |
| Isotype | Mouse IgG1 |
| Species Reactivity | Human |
| Tested Applications | WB |
| Recommended Dilution for WB | 1:500–1:5000 |
| Antigen | Recombinant epitope-Fc fusion protein |
| Epitope Sequence | TENSSQLDFEDVWNSSYGVNDS |
| Expression System | HEK293 cells |
| Purification | Protein G affinity chromatography |
| Conjugate | Unconjugated |
| Concentration | 0.1 mg/mL |
| Storage Buffer | 0.1 M Tris, 0.05 M Glycine, 0.07 M NaCl, 2 g/L BSA, 50% glycerol, pH 7 |
| Storage Condition | Store at -20°C. |
| Research Use | For research use only. Not for diagnostic or therapeutic applications. |
Product Background
Atypical chemokine receptor 1 (ACKR1), also known as Duffy antigen receptor for chemokines (DARC), is a seven-transmembrane atypical chemokine receptor involved in chemokine sequestration, inflammatory signaling regulation, leukocyte trafficking, vascular biology, and host-pathogen interactions.
ACKR1 is highly expressed in erythrocytes and endothelial cells and is widely studied in immunology, inflammation, infectious disease, hematology, vascular biology, and chemokine receptor signaling research.
Also known as: hACKR1, Human ACKR1, ACKR1, DARC, FY, CD234, Atypical chemokine receptor 1, Duffy antigen receptor for chemokines, Duffy antigen receptor, and Duffy blood group receptor.
Antibody Development
HM0062 was generated by phage-display selection using a mouse-derived antibody library. Recombinant epitope-Fc fusion protein containing the ACKR1 epitope sequence was used as the selection antigen. Following identification of the initial antibody candidate, structure-guided affinity optimization was performed to improve binding affinity and specificity.
The optimized antibody was reconstructed as a full-length mouse IgG1 recombinant antibody and expressed in HEK293 cells.
Binding and specificity were evaluated by:
- ELISA using recombinant epitope-Fc fusion antigen
- Western blot using recombinant MBP-epitope fusion protein
- Western blot detection of endogenous human ACKR1 protein in human cell lines
Validation Data
ELISA Validation
ELISA demonstrated specific binding to recombinant epitope-Fc fusion antigen, while minimal binding was observed against Fc control protein.
Validation conditions:
- Well 1: Fc control protein
- Well 2: Epitope-Fc fusion protein
- Detection substrate: TMB
Note:
ELISA validation confirms binding to the epitope-Fc fusion antigen. Full-length ACKR1 protein binding in solution has not been tested by ELISA.
Western Blot Validation – Recombinant Fusion Protein
Western blot analysis demonstrated specific recognition of recombinant MBP-epitope fusion protein expressed in BL21(DE3) cells, while no significant binding was observed against MBP control protein.
Validation conditions:
- Lane 1: MBP control protein
- Lane 2: MBP-epitope fusion protein
- Standard reducing Western blot
Western Blot Validation – Endogenous Full-Length Protein
Western blot analysis detected endogenous human ACKR1 protein in human cell lines under reducing conditions.
Validated samples:
- 293T cells
- HepG2 cells
Detection condition:
- Standard reducing Western blot
- HRP-conjugated anti-mouse secondary antibody
Observed bands:
- Higher molecular weight bands may represent protein glycosylation, oligomerization, or post-translationally modified species.
| Size | 20 ul, 100 ul |
|---|
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