Anti-mouse/human Notch1 Recombinant Monoclonal Antibody (XM0053)
Price range: $180.00 through $480.00
Anti-mouse/human Notch1 Recombinant Monoclonal Antibody (XM0053) is a high-affinity recombinant monoclonal antibody generated by phage-display selection from a mouse-derived antibody library, followed by structure-guided affinity optimization. The antibody recognizes both mouse and human Notch1 through a conserved epitope (AA 2188-2207) located within an intracellular flexible region of neurogenic locus notch homolog protein 1 (Notch1). It has been validated by ELISA using a recombinant epitope-Fc fusion antigen, by Western blot against a recombinant MBP-epitope fusion protein, by Western blot detection of endogenous Notch1 protein in HEK293T cell lysates and mouse tissues, and by immunofluorescence staining of mouse lung tissue.
Also known as: Notch1, Neurogenic locus notch homolog protein 1, TAN1, mNotch1, hNotch1.
For research use only. Not for diagnostic or therapeutic applications.
Product Information
| Property | Description |
|---|---|
| Product Name | Anti-mouse/human Notch1 Recombinant Monoclonal Antibody |
| Antibody ID | XM0053 |
| Target Protein | Neurogenic locus notch homolog protein 1 |
| Target Protein Short Name | Notch1 |
| UniProt Accession | P46531 (human), Q01705-1 (mouse), Q01705-2 (mouse), Q01705-3 (mouse), Q01705-4 (mouse) |
| Molecular Weight | Human: 272.5 kDa; Mouse isoform 1: 270.8 kDa, isoform 2: 237.4 kDa, isoform 3: 270.6 kDa, isoform 4: 269.2 kDa |
| Antibody Type | Recombinant Monoclonal Antibody |
| Host Species | Mouse |
| Recombinant Format | Full-length mouse IgG1 |
| Isotype | Mouse IgG1 |
| Species Reactivity | Mouse, Human |
| Tested Applications | WB, IF |
| Recommended Dilution for WB | 1:500–1:5000 |
| Recommended Dilution for IF | Optimize experimentally |
| Antigen | Recombinant epitope-Fc fusion protein |
| Epitope Sequence | SPVDSLESPHGYLSDVASPP |
| Expression System | HEK293 cells |
| Purification | Protein G affinity chromatography |
| Conjugate | Unconjugated |
| Concentration | 0.1 mg/mL |
| Storage Buffer | 0.1 M Tris, 0.05 M Glycine, 0.07 M NaCl, 2 g/L BSA, 50% glycerol, pH 7 |
| Storage Condition | Store at -20°C |
| Research Use | For research use only. Not for diagnostic or therapeutic applications. |
Product Background
Neurogenic locus notch homolog protein 1 (Notch1) is a core receptor in canonical Notch signaling and helps regulate cell-fate determination, differentiation, proliferation, and apoptotic programs. Notch1 is also linked to angiogenesis, thymocyte maturation, and multiple developmental signaling processes.
Notch1 is a valuable target for researchers studying developmental signaling, vascular biology, immunology, stem-cell regulation, differentiation programs, and cancer-related pathways.
Antibody Development
XM0053 was developed against a conserved Notch1 epitope (AA2198-2217). A recombinant epitope-Fc fusion protein was used as the selection antigen. After identification of the initial antibody candidate, structure-guided affinity optimization was performed to improve binding affinity and specificity.
XM0053 is supplied as a full-length mouse IgG1 recombinant antibody expressed in HEK293 cells.
Validation data include:
- ELISA using recombinant epitope-Fc fusion antigen
- Western blot using recombinant MBP-epitope fusion protein
- Western blot detection of endogenous Notch1 protein
- Immunofluorescence staining of endogenous mouse Notch1 in lung tissue
Validation Data
ELISA Validation
ELISA demonstrated specific binding to recombinant epitope-Fc fusion antigen, while minimal binding was observed against Fc control protein.
Validation conditions:
- Well 1: Fc control protein
- Well 2: Epitope-Fc fusion protein
- Detection substrate: TMB
Note:
ELISA validation confirms binding to the epitope-Fc fusion antigen. Full-length ADRA2A protein binding in solution has not been tested by ELISA.
Western Blot Validation – Recombinant Fusion Protein
Western blot analysis demonstrated specific recognition of recombinant MBP-epitope fusion protein expressed in BL21(DE3) cells, while no significant binding was observed against MBP control cell lysate.
Validation conditions:
- Lane 1: MBP control protein
- Lane 2: MBP-epitope fusion protein
- Standard reducing Western blot
Western Blot Validation – Endogenous Protein
Western blot analysis detected endogenous Notch1 protein under reducing conditions.
Validated samples:
- HEK293T cell
- Mouse Brain tissue
- Mouse Liver tissue
- Mouse Lung tissue
- Mouse Kidney tissue
Detection condition:
- Standard reducing Western blot
- HRP-conjugated anti-mouse secondary antibody
Observed bands:
(Left) Detection of Notch1 species in 293T cell lysates, brain, and liver tissues. Prominent bands in the HEK293T lane resolve the full-length precursor/PTM modified product (>200 kDa), the constitutive furin-cleaved transmembrane subunit (NTM, ~120 kDa), and the transcriptionally active Notch1 intracellular domain (NICD, ~80 kDa). (Right) Analysis in lung, kidney, and brain tissue lysates reveals highly tissue-specific, stable C-terminal processing fragments or degradation intermediates at ~50–55 kDa in lung and kidney, with an additional ~25 kDa fragment unique to kidney tissue. No detectable Notch1 signal was observed in adult brain tissue under either gel condition.
Immunofluorescence Validation
Immunofluorescence staining of mouse lung showed endogenous Notch1 signal using XM0053. Notch1 signal is shown in green and nuclei are counterstained with DAPI (blue).
| Size | 20 ul, 100 ul |
|---|
Related products
