Anti-mouse/human TRα Recombinant Monoclonal Antibody (XM0001)
Price range: $180.00 through $480.00
Anti-mouse/human TRα Recombinant Monoclonal Antibody (XM0001) is a high-affinity recombinant monoclonal antibody generated by phage-display selection from a mouse-derived antibody library, followed by structure-guided affinity optimization. The antibody recognizes both mouse and human TRα through a conserved epitope (AA 12-31) located within the N-terminal flexible region of thyroid hormone receptor alpha (TRα). It has been validated by ELISA using a recombinant epitope-Fc fusion antigen, by Western blot against a recombinant MBP-epitope fusion protein, by Western blot detection of endogenous TRα in HEK293T cells and mouse tissues, and by immunofluorescence staining of mouse kidney tissue.
Also known as: TRa, THRA, Thyroid hormone receptor alpha, Thyroid hormone receptor α, c-erbA-1, NR1A1, ERBA, Ear-1, Nuclear receptor subfamily 1 group A member 1.
For research use only. Not for diagnostic or therapeutic applications.
Product Information
| Item | Description |
|---|---|
| Product Name | Anti-mouse/humanTRα Recombinant Monoclonal Antibody |
| Catalog Number | XM0001 |
| Target | Thyroid hormone receptor alpha (TRα) |
| Gene Symbol | THRA |
| UniProt Accession |
Human: P10827-1 (54816Da), P10827-2 (46813Da), P10827-3 (50747Da), P10827-4 (50465Da); Mouse: P63058-1 (55023Da), P63058-2 (46795Da), P63058-3 (50954Da), P63058-4 (37926Da) |
| Host Species | Mouse |
| Recombinant Format | Full-length mouse IgG1 |
| Isotype | Mouse IgG1 |
| Species Reactivity | Mouse, Human |
| Immunogen | Recombinant epitope-Fc fusion protein |
| Epitope Sequence | SDPEENSARSPDGKRKRKN |
| Expression System | HEK293 cells |
| Purification | Protein G affinity chromatography |
| Concentration | 0.1 mg/mL |
| Tested Applications | WB, IF |
| Recommended WB Dilution | 1:500–1:5000 |
| Conjugate | Unconjugated |
| Storage Buffer | 0.1 M Tris, 0.05 M Glycine, 0.07 M NaCl, 2 g/L BSA, 50% Glycerol, pH 7.0 |
| Storage | Store at −20°C |
| Research Use | For research use only (RUO). Not for diagnostic or therapeutic applications. |
Product Background
Thyroid hormone receptor alpha (TRα), encoded by the THRA gene, is a member of the nuclear receptor superfamily that mediates the biological effects of thyroid hormones. TRα functions as a ligand-dependent transcription factor by binding thyroid hormone response elements (TREs) within target gene promoters, thereby regulating gene expression involved in development, metabolism, differentiation, cardiac function, skeletal muscle physiology, and central nervous system maturation. Multiple TRα isoforms are generated through alternative splicing, including the transcriptionally active TRα1 isoform and non-hormone-binding variants that modulate receptor signaling.
TRα is widely expressed in numerous tissues, with particularly high expression in the brain, heart, skeletal muscle, kidney, and gastrointestinal tract. Dysregulation of TRα signaling has been associated with developmental abnormalities, hypothyroidism, cardiovascular disease, metabolic disorders, and cancer. Because TRα regulates numerous physiological pathways, reliable antibodies are valuable tools for investigating receptor expression, tissue distribution, and thyroid hormone signaling mechanisms.
Validation Data
ELISA
XM0001 specifically recognizes the recombinant epitope-Fc fusion protein but shows no detectable binding to the Fc negative control, demonstrating high specificity toward the target epitope.
Western blot of recombinant proteins
XM0001 specifically recognizes the recombinant MBP-epitope fusion protein, while no signal is detected with MBP alone, confirming sequence-specific recognition of the TRα epitope.
Western blot of endogenous proteins
XM0001 detects endogenous TRα in HEK293T overexpression lysates as well as in mouse heart, brain, and kidney tissues. Multiple immunoreactive bands are observed, consistent with the presence of several TRα isoforms and post-translationally modified forms.
Immunofluorescence
XM0001 labels endogenous TRα in mouse kidney sections. Strong immunofluorescence is detected in renal tubular epithelial cells, consistent with the reported tissue distribution of thyroid hormone receptor alpha.
| Size | 20 ul, 100 ul |
|---|
Related products
