Anti-mouse/human RORγ Recombinant Monoclonal Antibody (XM0026)
Price range: $180.00 through $480.00
Anti-mouse/human RORγ Recombinant Monoclonal Antibody (XM0026) is a high-affinity recombinant monoclonal antibody generated by phage-display selection from a mouse-derived antibody library, followed by structure-guided affinity optimization. The antibody recognizes both mouse and human RORγ through a conserved epitope (AA 106–124) located within the flexible linker between the DNA-binding domain (DBD) and ligand-binding domain (LBD) of retinoid-related orphan receptor gamma (RORγ). It has been validated by ELISA using a recombinant epitope-Fc fusion antigen, by Western blot against a recombinant MBP-epitope fusion protein, by Western blot detection of endogenous and overexpressed full-length RORγ proteins in HEK293T cell lysates, and by immunofluorescence staining of mouse lymph node tissue.
Also known as: RORg, RORγ, ROR gamma, RORC, and RAR-related orphan receptor gamma.
For research use only. Not for diagnostic or therapeutic applications.
Product Information
| Property | Description |
|---|---|
| Product Name | Anti-mouse/human RORγ Recombinant Monoclonal Antibody |
| Antibody ID | XM0026 |
| Target Protein | Nuclear receptor ROR-gamma (RORγ / RORC) |
| UniProt Accession | Human: P51449-1 / P51449-2; Mouse: P51450-1 / P51450-2 / P51450-3 |
| Molecular Weight | Human isoforms: 55.8–58.2 kDa; Mouse isoforms: 38.5–58.1 kDa |
| Antibody Type | Recombinant monoclonal antibody |
| Host Species | Mouse |
| Recombinant Format | Full-length mouse IgG1 |
| Isotype | Mouse IgG1 |
| Species Reactivity | Mouse and human |
| Tested Applications | WB, IF, ELISA |
| Recommended Dilution for WB | 1:500–1:5000 |
| Antigen | Recombinant epitope-Fc fusion protein |
| Epitope Sequence | MSKKQRDSLHAEVQKQLQQ |
| Expression System | HEK293 cells |
| Purification | Protein G affinity chromatography |
| Conjugate | Unconjugated |
| Concentration | 0.1 mg/mL |
| Storage Buffer | 0.1 M Tris, 0.05 M Glycine, 0.07 M NaCl, 2 g/L BSA, 50% glycerol, pH 7 |
| Storage Condition | Store at -20°C. |
| Research Use | For research use only. Not for diagnostic or therapeutic applications. |
Product Background
Retinoic acid receptor-related orphan receptor gamma (RORγ / RORC) is a ligand-dependent nuclear receptor involved in immune regulation, Th17 cell differentiation, inflammation, metabolism, circadian rhythm regulation, and transcriptional control. RORγ is widely studied in immunology, autoimmune diseases, cancer immunology, metabolism, and nuclear receptor signaling research.
Antibody Development
XM0026 was generated by phage-display selection using a mouse-derived antibody library. Recombinant epitope-Fc fusion protein containing the RORγ epitope sequence was used as the selection antigen. Following identification of the initial antibody candidate, structure-guided affinity optimization was performed to improve binding affinity and specificity.
The optimized antibody was reconstructed as a full-length mouse IgG1 recombinant antibody and expressed in HEK293 cells.
Binding and specificity were evaluated by:
- ELISA using recombinant epitope-Fc fusion antigen
- Western blot using recombinant MBP-epitope fusion protein
- Western blot detection of overexpressed full-length RORγ protein
- Immunofluorescence staining of endogenous mouse RORγ in lymph node tissue
Validation Data
ELISA Validation
ELISA demonstrated specific binding to recombinant epitope-Fc fusion antigen, while minimal binding was observed against Fc control protein.
Validation conditions:
- Well 1: Fc control protein
- Well 2: Epitope-Fc fusion protein
- Detection substrate: TMB
Note:
ELISA validation confirms binding to the epitope-Fc fusion antigen. Full-length RORγ protein binding in solution has not been tested by ELISA.
Western Blot Validation – Recombinant Fusion Protein
Western blot analysis demonstrated specific recognition of recombinant MBP-epitope fusion protein expressed in BL21(DE3) cells, while no significant binding was observed against MBP control protein.
Validation conditions:
- Lane 1: MBP control protein
- Lane 2: MBP-epitope fusion protein
- Standard reducing Western blot
Western Blot Validation – Full-Length Protein
Western blot analysis confirmed recognition of overexpressed full-length RORγ protein under reducing conditions.
Validated samples:
- Overexpressed RORγ protein
- 293T cells
Detection condition:
- Standard reducing Western blot
- HRP-conjugated anti-mouse secondary antibody
Immunofluorescence Validation
Immunofluorescence staining of mouse lymph node tissue showed endogenous RORγ-associated signal using XM0026. RORγ signal is shown in green and nuclei are counterstained with DAPI (blue).
| Size | 20 ul, 100 ul |
|---|
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