Anti-human GPR25 (Extracellular) Recombinant Monoclonal Antibody (HM0063)
Price range: $230.00 through $720.00
Anti-human GPR25 Recombinant Monoclonal Antibody (HM0063) is a high-affinity recombinant monoclonal antibody generated by phage-display selection from a mouse-derived antibody library, followed by structure-guided affinity optimization. The antibody recognizes an epitope (AA 1–22) located within the N-terminal extracellular flexible region of G protein-coupled receptor 25 (GPR25). It has been validated by ELISA using a recombinant epitope-Fc fusion antigen, by Western blot against a recombinant MBP-epitope fusion protein, and by Western blot detection of endogenous GPR25 protein in human cell lysates.
Also known as: hGPR25, GPR25, and C-X-C chemokine receptor GPR25.
For research use only. Not for diagnostic or therapeutic applications.
Product Information
| Property | Description |
|---|---|
| Product Name | Anti-human GPR25 Recombinant Monoclonal Antibody |
| Antibody ID | HM0063 |
| Target Protein | C-X-C chemokine receptor GPR25 |
| UniProt Accession | Human: O00155 |
| Molecular Weight | Human: 38.8 kDa |
| Antibody Type | Recombinant monoclonal antibody |
| Host Species | Mouse |
| Recombinant Format | Full-length mouse IgG1 |
| Isotype | Mouse IgG1 |
| Species Reactivity | Human |
| Tested Applications | WB |
| Recommended Dilution for WB | 1:500–1:5000 |
| Antigen | Recombinant epitope-Fc fusion protein |
| Epitope Sequence | MAPTEPWSPSPGSAPWDYSGLD |
| Expression System | HEK293 cells |
| Purification | Protein G affinity chromatography |
| Conjugate | Unconjugated |
| Concentration | 0.1 mg/mL |
| Storage Buffer | 0.1 M Tris, 0.05 M Glycine, 0.07 M NaCl, 2 g/L BSA, 50% glycerol, pH 7 |
| Storage Condition | Store at -20°C. |
| Research Use | For research use only. Not for diagnostic or therapeutic applications. |
Product Background
GPR25 is an orphan G protein-coupled receptor (GPCR) belonging to the chemokine receptor family. GPR25 is involved in immune regulation, chemokine signaling, cell migration, inflammatory responses, and GPCR-mediated signaling pathways.
GPR25 is studied in immunology, inflammation, receptor biology, signal transduction, cancer biology, and chemokine receptor research.
Antibody Development
HM0063 was generated by phage-display selection using a mouse-derived antibody library. Recombinant epitope-Fc fusion protein containing the GPR25 epitope sequence was used as the selection antigen. Following identification of the initial antibody candidate, structure-guided affinity optimization was performed to improve binding affinity and specificity.
The optimized antibody was reconstructed as a full-length mouse IgG1 recombinant antibody and expressed in HEK293 cells.
Binding and specificity were evaluated by:
- ELISA using recombinant epitope-Fc fusion antigen
- Western blot using recombinant MBP-epitope fusion protein
- Western blot detection of endogenous human GPR25 protein
Validation Data
ELISA Validation
ELISA demonstrated specific binding to recombinant epitope-Fc fusion antigen, while minimal binding was observed against Fc control protein.
Validation conditions:
- Well 1: Fc control protein
- Well 2: Epitope-Fc fusion protein
- Detection substrate: TMB
Note:
ELISA validation confirms binding to the epitope-Fc fusion antigen. Full-length GPR25 protein binding in solution has not been tested by ELISA.
Western Blot Validation – Recombinant Fusion Protein
Western blot analysis demonstrated specific recognition of recombinant MBP-epitope fusion protein expressed in BL21(DE3) cells, while no significant binding was observed against MBP control protein.
Validation conditions:
- Lane 1: MBP control protein
- Lane 2: MBP-epitope fusion protein
- Standard reducing Western blot
Western Blot Validation – Endogenous Full-Length Protein
Western blot analysis detected endogenous human GPR25 protein in human cell lines under reducing conditions.
Validated samples:
- 293T cells
- HeLa cells
- HepG2 cells
Detection condition:
- Standard reducing Western blot
- HRP-conjugated anti-mouse secondary antibody
Observed bands:
- Major bands observed near expected GPR25 molecular weight
| Size | 20 ul, 100 ul |
|---|
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