Anti-human GPR84 Recombinant Monoclonal Antibody (HM0061)
Price range: $230.00 through $720.00
Anti-human GPR84 Recombinant Monoclonal Antibody (HM0061) is a high-affinity recombinant monoclonal antibody generated by phage-display selection from a mouse-derived antibody library, followed by structure-guided affinity optimization. The antibody recognizes an epitope (AA 277-298) located within the intracellular flexible region of G protein-coupled receptor 84 (GPR84). It has been validated by ELISA using a recombinant epitope-Fc fusion antigen, by Western blot against a recombinant MBP-epitope fusion protein, and by Western blot detection of endogenous GPR84 protein in human cell lysates.
Also known as: hGPR84, GPR84, EX33, and G-protein coupled receptor 84.
For research use only. Not for diagnostic or therapeutic applications.
Product Information
| Property | Description |
|---|---|
| Product Name | Anti-human GPR84 Recombinant Monoclonal Antibody |
| Antibody ID | HM0061 |
| Target Protein | G-protein coupled receptor 84 |
| Target Protein Short Name | hGPR84 |
| UniProt Accession | Q9NQS5 |
| Molecular Weight | Human: 43.7 kDa |
| Antibody Type | Recombinant monoclonal antibody |
| Host Species | Mouse |
| Recombinant Format | Full-length mouse IgG1 |
| Isotype | Mouse IgG1 |
| Species Reactivity | Human |
| Tested Applications | WB |
| Recommended Dilution for WB | 1:500–1:5000 |
| Antigen | Recombinant epitope-Fc fusion protein |
| Epitope Sequence | QINSKRAKQMAEKSPPEASAKA |
| Expression System | HEK293 cells |
| Purification | Protein G affinity chromatography |
| Conjugate | Unconjugated |
| Concentration | 0.1 mg/mL |
| Storage Buffer | 0.1 M Tris, 0.05 M Glycine, 0.07 M NaCl, 2 g/L BSA, 50% glycerol, pH 7 |
| Storage Condition | Store at -20°C. |
| Research Use | For research use only. Not for diagnostic or therapeutic applications. |
Product Background
G-protein coupled receptor 84 (GPR84) is an immune-associated G protein-coupled receptor involved in inflammatory signaling, innate immune responses, macrophage biology, metabolic inflammation, and receptor-mediated signal transduction. GPR84 has been studied in inflammation, immune regulation, fibrosis, metabolic disease, and cancer-related signaling pathways.
GPR84 is a useful target for researchers studying GPCR biology, immunology, inflammatory disease mechanisms, receptor pharmacology, and immune-metabolic signaling.
Antibody Development
HM0061 was generated by phage-display selection using a mouse-derived antibody library. A recombinant epitope-Fc fusion protein containing the GPR84 epitope sequence was used as the selection antigen. After identification of the initial antibody candidate, structure-guided affinity optimization was performed to improve binding affinity and specificity.
The optimized antibody was reconstructed as a full-length mouse IgG1 recombinant antibody and expressed in HEK293 cells.
Binding and specificity were evaluated by:
- ELISA using recombinant epitope-Fc fusion antigen
- Western blot using recombinant MBP-epitope fusion protein
- Western blot detection of endogenous human GPR84 protein
Validation Data
ELISA Validation
ELISA demonstrated specific binding to recombinant epitope-Fc fusion antigen, while minimal binding was observed against Fc-only control protein.
Validation conditions:
- Fc control protein
- Epitope-Fc fusion protein
- Detection substrate: TMB
Note: ELISA validation confirms binding to epitope-Fc fusion antigen. Full-length GPR84 protein binding in solution has not been tested by ELISA.
Western Blot Validation – Recombinant Fusion Protein
Western blot analysis confirmed specific recognition of recombinant MBP-epitope fusion protein, with minimal signal against MBP control protein.
Validation conditions:
- MBP control protein
- MBP-epitope fusion protein
- Standard reducing Western blot
Western Blot Validation – Endogenous Full-Length Protein
Western blot analysis detected endogenous human GPR84 protein in human cell lysates under reducing conditions.
Validated samples:
- 293T cells
- HeLa cells
- HepG2 cells
Observed bands:
- Major immunoreactive bands were detected around 45–50 kDa, consistent with the expected molecular weight range of human GPR84.
- Additional lower molecular weight bands may represent processed receptor fragments, degradation products, alternatively modified receptor forms, or GPCR-associated species.
| Size | 20 ul, 100 ul |
|---|
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