Product Information

Property	Description
Product Name	Anti-mouse/human VDR Recombinant Monoclonal Antibody
Antibody ID	XM0018
Target Protein	Vitamin D receptor (VDR)
UniProt Accession	Mouse: P48281; Human: P11473-1 / P11473-2
Molecular Weight	Mouse: 47.8 kDa; Human isoforms: 48.3–53.9 kDa
Antibody Type	Recombinant monoclonal antibody
Host Species	Mouse
Recombinant Format	Full-length mouse IgG1
Isotype	Mouse IgG1
Species Reactivity	Mouse and human validated
Tested Applications	WB, IF, ELISA
Recommended Dilution for WB	1:500–1:5000
Antigen	Recombinant epitope-Fc fusion protein
Epitope Sequence	MEAMAASTSLPDPGDFDR
Expression System	HEK293 cells
Purification	Protein G affinity chromatography
Conjugate	Unconjugated
Concentration	0.1 mg/mL
Storage Buffer	0.1 M Tris, 0.05 M Glycine, 0.07 M NaCl, 2 g/L BSA, 50% glycerol, pH 7
Storage Condition	Store at -20°C
Research Use	For research use only. Not for diagnostic or therapeutic applications.

Product Background

Vitamin D3 receptor (VDR, NR1I1) is a ligand-activated nuclear receptor that mediates vitamin D signaling and regulates calcium homeostasis, immune responses, metabolism, differentiation, and transcriptional regulation. VDR is widely studied in bone biology, endocrinology, immunology, cancer biology, inflammation, kidney biology, and metabolic disease research.

Antibody Development

XM0018 was generated by phage-display selection using a mouse-derived antibody library. Recombinant epitope-Fc fusion protein containing the VDR epitope sequence was used as the selection antigen. Following identification of the initial antibody candidate, structure-guided affinity optimization was performed to improve binding affinity and specificity.

The optimized antibody was reconstructed as a full-length mouse IgG1 recombinant antibody and expressed in HEK293 cells.

Binding and specificity were evaluated by:

• ELISA using recombinant epitope-Fc fusion antigen
• Western blot using recombinant MBP-epitope fusion protein
• Western blot detection of endogenous VDR protein in mouse tissues and human cells
• Immunofluorescence staining of endogenous mouse VDR in intestine tissue

Validation Data

ELISA Validation

ELISA demonstrated specific binding to recombinant epitope-Fc fusion antigen, while minimal binding was observed against Fc control protein.

Validation conditions:

• Well 1: Fc control protein
• Well 2: Epitope-Fc fusion protein
• Detection substrate: TMB

Note: ELISA validation confirms binding to the epitope-Fc fusion antigen. Full-length VDR protein binding in solution has not been tested by ELISA.

Western Blot Validation – Recombinant Fusion Protein

Western blot analysis demonstrated specific recognition of recombinant MBP-epitope fusion protein expressed in BL21(DE3) cells, while no significant binding was observed against MBP control protein.

Validation conditions:

• Lane 1: MBP control protein
• Lane 2: MBP-epitope fusion protein
• Standard reducing Western blot

Western Blot Validation – Endogenous Full-Length Protein

Western blot analysis detected endogenous VDR protein in human 293T cells and mouse intestine and kidney tissues under reducing conditions.

Validated samples:

• 293T cells
• Mouse intestine tissue
• Mouse kidney tissue

Detection condition:

• Standard reducing Western blot
• HRP-conjugated anti-mouse secondary antibody

Immunofluorescence Validation

Immunofluorescence staining of mouse intestine tissue showed endogenous VDR-associated signal using XM0018. VDR signal is shown in green and nuclei are counterstained with DAPI (blue).