Anti-human EDNRB Recombinant Monoclonal Antibody (HM0081)
Price range: $230.00 through $720.00
Anti-human EDNRB Recombinant Monoclonal Antibody (HM0081) is a high-affinity recombinant monoclonal antibody generated by phage-display selection from a mouse-derived antibody library followed by structure-guided affinity optimization. The antibody was validated by ELISA against recombinant epitope-Fc fusion antigen and by Western blot detection of endogenous EDNRB protein.
Also known as: hEDNRB, EDNRB, ETBR, and Endothelin receptor type B.
For research use only. Not for diagnostic or therapeutic applications.
Product Information
| Property | Description |
|---|---|
| Product Name | Anti-human EDNRB Recombinant Monoclonal Antibody |
| Antibody ID | HM0081 |
| Target Protein | Endothelin receptor type B |
| Target Protein Short Name | EDNRB |
| UniProt Accession | P24530-1, P24530-2, P24530-3 |
| Molecular Weight | Human isoforms: 49.6 kDa, 48.7 kDa, 59.5 kDa |
| Antibody Type | Recombinant monoclonal antibody |
| Host Species | Mouse |
| Recombinant Format | Full-length mouse IgG1 |
| Isotype | Mouse IgG1 |
| Species Reactivity | Human |
| Tested Applications | WB |
| Recommended Dilution for WB | 1:500–1:5000 |
| Antigen | Recombinant epitope-Fc fusion protein |
| Epitope Sequence | GSNASLARSLAPAEVPKGDRTA |
| Expression System | HEK293 cells |
| Purification | Protein G affinity chromatography |
| Conjugate | Unconjugated |
| Concentration | 0.1 mg/mL |
| Storage Buffer | 0.1 M Tris, 0.05 M Glycine, 0.07 M NaCl, 2 g/L BSA, 50% glycerol, pH 7 |
| Storage Condition | Store at -20°C |
| Research Use | For research use only. Not for diagnostic or therapeutic applications |
Product Background
EDNRB (Endothelin receptor type B) is a G protein-coupled receptor that binds endothelins and mediates diverse physiological functions including vascular tone regulation, neural crest development, melanocyte biology, gastrointestinal function, and cell signaling. EDNRB has important roles in cardiovascular biology, cancer research, developmental biology, neural signaling, and GPCR-mediated signaling pathways.
EDNRB is widely studied in vascular biology, oncology, neurobiology, melanocyte biology, GPCR signaling, endothelin signaling pathways, and receptor pharmacology.
Antibody Development
HM0081 was generated by phage-display selection using a mouse-derived antibody library. Recombinant epitope-Fc fusion protein containing the EDNRB epitope sequence was used as the selection antigen. Following identification of the initial antibody candidate, structure-guided affinity optimization was performed to improve binding affinity and specificity.
The optimized antibody was reconstructed as a full-length mouse IgG1 recombinant antibody and expressed in HEK293 cells.
Binding and specificity were evaluated by:
- ELISA using recombinant epitope-Fc fusion antigen
- Western blot detection of endogenous full-length EDNRB protein
Validation Data
ELISA Validation
ELISA demonstrated specific binding to recombinant epitope-Fc fusion antigen, while minimal binding was observed against Fc control protein.
Validation conditions:
- Well 1: Fc control protein
- Well 2: Epitope-Fc fusion protein
- Detection substrate: TMB
Note:
ELISA validation confirms binding to the epitope-Fc fusion antigen. Full-length EDNRB protein binding in solution has not been tested by ELISA.
Western Blot Validation – Endogenous Full-Length Protein
Western blot analysis detected endogenous full-length EDNRB protein under reducing conditions.
Validated samples:
- 293T cells
- HepG2 cells
Detection condition:
- Standard reducing Western blot
- HRP-conjugated anti-mouse secondary antibody
Observed bands:
- Major bands observed near the expected molecular weight range for endogenous EDNRB isoforms
- Additional lower molecular weight bands may represent processed receptor fragments, degradation products, alternatively modified receptor forms, or GPCR-associated species.
.
| Size | 20 ul, 100 ul |
|---|
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